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Biological Indicators for Use in Isolator Decontamination Applications by James R. Rickloff, M.S. , Principal Scientist 8-Mar-01
This paper reviews the issues that isolator users need to be aware of prior to specifying and using a biological indicator for their decontamination cycle validation studies.
Resistance of Various Microorganisms To Vaporized Hydrogen Peroxide In A Prototype Table Top Sterilizer by James R. Rickloff, M.S., and Paula Orelski, American Sterilizer Company, Erie, PA, Proceedings of the 89th Annual Meeting of the American Society for Microbiology 1989
A great deal of information is available in the literature on the relative resistance of microorganisms to aqueous hydrogen peroxide (H202); however, the same cannot be said for H202 in the gaseous state. Bacillus stearothermophilus spores were found to be the most resistant to the sterilant and the presence of organic soil did not affect the ability of H202 gas to sterilize stainless surfaces.
D-value Determination by R. Chris Smith, BS, Validation Scientist 29-Mar-01
This paper covers the factors that can affect a D-value and it discusses the equipment and methods that have been used to satisfactorily estimate it within isolator applications.




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